Faculty Collaboration Database

Mutational specificities of environmental carcinogens in the lacI gene of Escherichia coli: IV. The tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Carcinogenesis 1991 Feb;12(2):221-4

Date

02/01/1991

Pubmed ID

1995189

DOI

10.1093/carcin/12.2.221

Scopus ID

2-s2.0-0026096959 (requires institutional sign-in at Scopus site)   15 Citations

Abstract

We have determined the mutational specificity of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) by the characterization of 58 induced Escherichia coli lacI-d mutants at the DNA sequence level. Metabolic activation of NNK was carried out using the S9 fraction from Aroclor 1254-treated rats. G:C----A:T transitions dominated the spectrum, accounting for 55% of the mutations recovered. The other base substitutions recovered include three A:T----G:C transitions as well as two A:T----T:A, three A:T----C:G, five G:C----C:G and five G:C----T:A transversions. Other classes of mutational events included two deletions, three duplications and three frame-shifts. The complexity of the NNK mutational spectrum appears consistent with a model that this compound induces mutations by both the methylation and the pyridoloxobutylation of DNA.

Author List

Jiao JL, Zielenska M, Anderson MW, Glickman BW

MESH terms used to index this publication - Major topics in bold

Bacterial Proteins
Base Sequence
Carcinogens
Environmental Exposure
Escherichia coli
Escherichia coli Proteins
Lac Repressors
Molecular Sequence Data
Mutation
Nitrosamines
Repressor Proteins