Faculty Collaboration Database

Epigenomics: maternal high-fat diet exposure in utero disrupts peripheral circadian gene expression in nonhuman primates. FASEB J 2011 Feb;25(2):714-26

Date

11/26/2010

Pubmed ID

21097519

Pubmed Central ID

PMC3228348

DOI

10.1096/fj.10-172080

Scopus ID

2-s2.0-79551627622 (requires institutional sign-in at Scopus site)   116 Citations

Abstract

The effect of in utero exposure to a maternal high-fat diet on the peripheral circadian system of the fetus is unknown. Using mRNA copy number analysis, we report that the components of the peripheral circadian machinery are transcribed in the nonhuman primate fetal liver in an intact phase-antiphase fashion and that Npas2, a paralog of the Clock transcription factor, serves as the rate-limiting transcript by virtue of its relative low abundance (10- to 1000-fold lower). We show that exposure to a maternal high-fat diet in utero significantly alters the expression of fetal hepatic Npas2 (up to 7.1-fold, P<0.001) compared with that in control diet-exposed animals and is reversible in fetal offspring from obese dams reversed to a control diet (1.3-fold, P>0.05). Although the Npas2 promoter remains largely unmethylated, differential Npas2 promoter occupancy of acetylation of fetal histone H3 at lysine 14 (H3K14ac) occurs in response to maternal high-fat diet exposure compared with control diet-exposed animals. Furthermore, we find that disruption of Npas2 is consistent with high-fat diet exposure in juvenile animals, regardless of in utero diet exposure. In summary, the data suggest that peripheral Npas2 expression is uniquely vulnerable to diet exposure.

Author List

Suter M, Bocock P, Showalter L, Hu M, Shope C, McKnight R, Grove K, Lane R, Aagaard-Tillery K

MESH terms used to index this publication - Major topics in bold

Animals
Circadian Rhythm
Dietary Fats
Disease Models, Animal
Epigenomics
Female
Gene Expression Profiling
Gene Expression Regulation
Liver
Macaca
Maternal Nutritional Physiological Phenomena
Nerve Tissue Proteins
Pregnancy
Prenatal Exposure Delayed Effects
RNA, Messenger
Transcription Factors