Chronic exposure to morphine produces a marked cardioprotective phenotype in aged mouse hearts. Exp Gerontol 2004 Jul;39(7):1021-6
Date
07/09/2004Pubmed ID
15236761DOI
10.1016/j.exger.2004.03.038Scopus ID
2-s2.0-3242656401 (requires institutional sign-in at Scopus site) 63 CitationsAbstract
Aging is often associated with decreased myocardial ischemic tolerance. We recently reported that chronic preconditioning produced by continuous exposure to morphine affords a profound cardioprotective phenotype in young mice. In this study, we determined if chronic exposure to morphine retained its ability to precondition the myocardium in the young or aged heart. Young (10-14 weeks) or aged (24-26 months) C57/BL6 mice were untreated, administered morphine acutely (30 microM), or implanted with a morphine pellet (75 mg) for 5 days prior to heart isolation and perfusion. Following equilibration, perfused hearts were subjected to 25 min ischemia and 45 min reperfusion. Untreated hearts from both young and aged mice displayed marked contractile dysfunction and LDH release following reperfusion. Acute infusion of morphine improved recovery of end-diastolic pressure and developed pressure in young (P < 0.05 vs. untreated) but not senescent hearts. Hearts from mice exposed to morphine for 5 days displayed a further improvement in post-ischemic contractile function (P < 0.05 vs. acute treatment), and a marked reduction in post-ischemic LDH efflux (P < 0.05 vs. untreated) in both young and senescent hearts. These data demonstrate that aged hearts maintain the ability to be preconditioned by chronic exposure to morphine in the absence of acute protection.
Author List
Peart JN, Gross GJMESH terms used to index this publication - Major topics in bold
AgingAnimals
Drug Administration Schedule
Hemodynamics
Ischemic Preconditioning, Myocardial
L-Lactate Dehydrogenase
Male
Mice
Mice, Inbred C57BL
Morphine
Myocardial Contraction
Myocardial Reperfusion
Myocardial Reperfusion Injury
Organ Culture Techniques
