Faculty Collaboration Database

Sarcolemmal KATP channel triggers delayed ischemic preconditioning in rats. Am J Physiol Heart Circ Physiol 2005 Jan;288(1):H445-7

Date

11/26/2004

Pubmed ID

15563543

DOI

10.1152/ajpheart.00031.2004

Scopus ID

2-s2.0-16644388956 (requires institutional sign-in at Scopus site)   54 Citations

Abstract

Previous work from our laboratory has shown that the sarcolemmal K(ATP) channel (sK(ATP)) is required as a trigger for delayed cardioprotection upon exogenous opioid administration. We also established that the mitochondrial K(ATP) (mK(ATP)) channel is not required for triggering delayed delta-opioid-induced infarct size reduction. Because mechanistic differences have been found among delta-opioids and that due to ischemic preconditioning (IPC), we determined whether the triggering mechanism of delayed IPC-induced infarct size reduction involves either the sK(ATP) or mK(ATP). Male Sprague-Dawley rats received either sham surgery or IPC (3- to 5-min cycles of ischemia and reperfusion) 24 h before being subjected to 30 min of ischemia and 2 h of reperfusion. Infarct size was determined and expressed as a percentage of the area at risk, with significance compared with sham reported at P </= 0.001. A subset of both sham and IPC-treated rats received either the selective sK(ATP) channel antagonist, HMR-1098 (6 mg/kg), or the selective mK(ATP) channel antagonist, 5-hydroxydeconoic acid (5-HD; 10 mg/kg), given 5 min before IPC. Rats subjected to IPC demonstrated a significant reduction in infarct size compared with sham (29.2 +/- 4.7 vs. 59.3 +/- 2.5%, respectively; P </= 0.001). Prior administration of HMR-1098, but not 5-HD, abolished IPC-induced infarct size reduction (48.8 +/- 2.9 and 28.8 +/- 4.0%, respectively; P </= 0.001). Furthermore, administration of HMR 24 h after IPC, before index ischemia, did not abrogate IPC-induced infarct size reduction (33.0 +/- 5.0 vs. 29.2 +/- 4.7%, respectively; P </= 0.001). These data suggest that the sK(ATP) channel is required as a trigger but not a mediator for delayed IPC-induced infarct size reduction in rat hearts.

Author List

Patel HH, Gross ER, Peart JN, Hsu AK, Gross GJ

MESH terms used to index this publication - Major topics in bold

Adenosine Triphosphate
Animals
Benzamides
Decanoic Acids
Hemodynamics
Hydroxy Acids
Ischemic Preconditioning, Myocardial
Male
Mitochondria, Heart
Myocardial Infarction
Myocardium
Potassium Channel Blockers
Potassium Channels
Rats
Rats, Sprague-Dawley
Sarcolemma
Time Factors