Cardioprotective effects of 70-kDa heat shock protein in transgenic mice. Proc Natl Acad Sci U S A 1996 Mar 19;93(6):2339-42
Date
03/19/1996Pubmed ID
8637874Pubmed Central ID
PMC39797DOI
10.1073/pnas.93.6.2339Scopus ID
2-s2.0-9244263563 (requires institutional sign-in at Scopus site) 250 CitationsAbstract
Heat shock proteins are proposed to limit injury resulting from diverse environmental stresses, but direct metabolic evidence for such a cytoprotective function in vertebrates has been largely limited to studies of cultured cells. We generated lines of transgenic mice to express human 70-kDa heat shock protein constitutively in the myocardium. Hearts isolated from these animals demonstrated enhanced recovery of high energy phosphate stores and correction of metabolic acidosis following brief periods of global ischemia sufficient to induce sustained abnormalities of these variables in hearts from nontransgenic littermates. These data demonstrate a direct cardioprotective effect of 70-kDa heat shock protein to enhance postischemic recovery of the intact heart.
Author List
Radford NB, Fina M, Benjamin IJ, Moreadith RW, Graves KH, Zhao P, Gavva S, Wiethoff A, Sherry AD, Malloy CR, Williams RSAuthor
Ivor J. Benjamin MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Adenine NucleotidesAnimals
Energy Metabolism
HSP70 Heat-Shock Proteins
Humans
Magnetic Resonance Spectroscopy
Mice
Mice, Transgenic
Myocardium
Reperfusion Injury
