Metal ions activate vascular endothelial cells and increase lymphocyte chemotaxis and binding. J Orthop Res 2013 Sep;31(9):1484-91
Date
05/01/2013Pubmed ID
23629852Pubmed Central ID
PMC3957232DOI
10.1002/jor.22377Scopus ID
2-s2.0-84881022485 (requires institutional sign-in at Scopus site) 23 CitationsAbstract
Metal on metal articulations in hip arthroplasty offer advantages, including lower volumetric wear compared to conventional metalonpolyethylene bearings, and increased resistance to dislocation. Reports described early failures, with histologic features similar to a Type IV immune response. Mechanisms by which metal wear products cause this reaction are not completely understood. We hypothesized a mechanism through direct activation of endothelial cells (ECs) by metal ions, resulting in both vasculitis and accumulation of lymphocytes without prior immune sensitization. Effects of metal ions were evaluated using human ECs in culture. Alterations in chemotactic proteins IL8 and MCP1 were assessed, as was upregulation of the adhesion molecule ICAM-1 and lymphocyte binding to ECs. Cobalt increased secretion of IL8 and MCP1 significantly, and upregulated the expression of ICAM-1 in ECs compared to stimulation by chromium and controls. Binding of lymphocytes to ECs and transEC migration were both significantly increased by cobalt but not chromium. These findings suggest that cobalt contributes more to the activation of ECs and lymphocyte binding than chromium without an allergic response. Some of the adverse tissue reactions to implants with components made of cobalt-chromium-molybdenium alloys may be due in part to activation of the endothelium by metal ions.
Author List
Ninomiya JT, Kuzma SA, Schnettler TJ, Krolikowski JG, Struve JA, Weihrauch DMESH terms used to index this publication - Major topics in bold
Blotting, WesternCell Adhesion
Cell Survival
Chemokine CCL2
Chemotaxis, Leukocyte
Chlorides
Chromium Compounds
Cobalt
Dose-Response Relationship, Drug
Human Umbilical Vein Endothelial Cells
Humans
Intercellular Adhesion Molecule-1
Interleukin-8
Ions
Jurkat Cells
Lymphocytes
Metal-on-Metal Joint Prostheses
Up-Regulation
