Tactile and Kinesthetic Stimulation (TKS) intervention improves outcomes in weanling rat bone in a neonatal stress model. J Musculoskelet Neuronal Interact 2013 Jun;13(2):157-65
Date
06/04/2013Pubmed ID
23728102Scopus ID
2-s2.0-84878579240 (requires institutional sign-in at Scopus site) 1 CitationAbstract
OBJECTIVES: Preterm infants are born with low bone mineral. Neonatal stress further impedes bone mineralization. Clinical evidence suggests that tactile and kinesthetic stimulation (TKS) improves bone phenotype and decreases stress response. Clinical and translational studies indicate the IGF-1 axis, responsible for postnatal growth and bone mineralization, is a key player. We hypothesized that TKS would attenuate the negative impact of neonatal stress on bone phenotype and the IGF-1 axis in weanling rats.
METHODS: Neonatal stress (STRESS) or TKS (STRESS + 10min TKS) was administered from D6 to D10. Control animals received standard care. Tissue was harvested on D21. Dual energy x-ray absorptiometry (DXA) and bone morphometry were performed and serum osteocalcin, type I procollagen N-terminal propeptide (PINP), tartrate-resistant acid phosphatase (TRAP), and bone and liver mRNA levels of IGF-1, IGF-1 receptor (IGF-1R), and growth hormone receptor (GHR) were measured.
RESULTS: Neonatal stress increased bone mineral content (BMC), area (BA), growth plate width, liver IGF-1 mRNA, and serum IGF-1. TKS maintained areal bone mineral density (aBMD) and bone specific IGF-1 and IGF-1R mRNA while STRESS decreased compared to controls.
CONCLUSIONS: Neonatal stress results in apparent accelerated growth response. TKS differed from STRESS with improved tibia aBMD and increased bone specific IGF-1 mRNA.
Author List
Haley S, Gulliver K, Baldassarre R, Miller S, Lane RH, Moyer-Mileur LJMESH terms used to index this publication - Major topics in bold
Absorptiometry, PhotonAnimals
Animals, Newborn
Biomarkers
Body Weight
Bone Development
Bone Resorption
Bone and Bones
Calcification, Physiologic
Densitometry
Epiphyses
Female
Growth Plate
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor I
Kinesthesis
Pregnancy
RNA, Messenger
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Stress, Psychological
Tibia
Touch
