Faculty Collaboration Database

Medulloblastoma cell line secretes platelet-derived growth factor. Pediatr Neurol 1989;5(6):347-52

Date

11/01/1989

Pubmed ID

2604798

DOI

10.1016/0887-8994(89)90047-7

Scopus ID

2-s2.0-0024831455 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

Medulloblastoma is the most common malignant childhood brain tumor in which aggressive growth produces recurrence in approximately 50% of appropriately treated cases and metastases along the neuraxis in 30%. To date, no studies exist concerning the production of autocrine growth factors by this brain tumor type. Malignant brain tumors in adults often produce platelet-derived growth factor (PDGF). A medulloblastoma cell line, TE-671, has been used for many years in pediatric neuro-oncologic studies. We assayed this medulloblastoma cell line for the production of PDGF. PDGF is produced by medulloblastoma cells grown in monolayer tissue culture and stimulates PDGF-sensitive 3T3 fibroblasts to incorporate tritiated thymidine in a dose-dependent fashion. This biologic activity is blocked by PDGF antibodies in a dose-dependent relationship. We postulate that PDGF produced by medulloblastoma cells plays a role in the growth of this tumor by stimulating mitogenic activity.

Author List

Whelan HT, Nelson DB, Strother D, Przybylski C, Figge G, Mamandi A

MESH terms used to index this publication - Major topics in bold

Humans
Immune Sera
Medulloblastoma
Platelet-Derived Growth Factor
Tumor Cells, Cultured