Genomic analysis of immune response against Vibrio cholerae hemolysin in Caenorhabditis elegans. PLoS One 2012;7(5):e38200
Date
06/08/2012Pubmed ID
22675448Pubmed Central ID
PMC3364981DOI
10.1371/journal.pone.0038200Scopus ID
2-s2.0-84861679962 (requires institutional sign-in at Scopus site) 46 CitationsAbstract
Vibrio cholerae cytolysin (VCC) is among the accessory V. cholerae virulence factors that may contribute to disease pathogenesis in humans. VCC, encoded by hlyA gene, belongs to the most common class of bacterial toxins, known as pore-forming toxins (PFTs). V. cholerae infects and kills Caenorhabditis elegans via cholerae toxin independent manner. VCC is required for the lethality, growth retardation and intestinal cell vacuolation during the infection. However, little is known about the host gene expression responses against VCC. To address this question we performed a microarray study in C. elegans exposed to V. cholerae strains with intact and deleted hlyA genes.Many of the VCC regulated genes identified, including C-type lectins, Prion-like (glutamine [Q]/asparagine [N]-rich)-domain containing genes, genes regulated by insulin/IGF-1-mediated signaling (IIS) pathway, were previously reported as mediators of innate immune response against other bacteria in C. elegans. Protective function of the subset of the genes up-regulated by VCC was confirmed using RNAi. By means of a machine learning algorithm called FastMEDUSA, we identified several putative VCC induced immune regulatory transcriptional factors and transcription factor binding motifs. Our results suggest that VCC is a major virulence factor, which induces a wide variety of immune response- related genes during V. cholerae infection in C. elegans.
Author List
Sahu SN, Lewis J, Patel I, Bozdag S, Lee JH, LeClerc JE, Cinar HNMESH terms used to index this publication - Major topics in bold
Amino Acid MotifsAnimals
Bacillus thuringiensis
Bacterial Proteins
Bacterial Toxins
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Gene Expression Profiling
Gene Expression Regulation
Genomics
Hemolysin Proteins
Immunity, Innate
Inflammation
RNA Interference
Temperature
Transcription, Genetic
Unfolded Protein Response
Vibrio cholerae
Virulence Factors
