Faculty Collaboration Database

Protein-protein interactions in the synaptonemal complex. Mol Biol Cell 1997 Aug;8(8):1405-14

Date

08/01/1997

Pubmed ID

9285814

Pubmed Central ID

PMC276165

DOI

10.1091/mbc.8.8.1405

Scopus ID

2-s2.0-0030982432 (requires institutional sign-in at Scopus site)   47 Citations

Abstract

In mammalian systems, an approximately M(r) 30,000 Cor1 protein has been identified as a major component of the meiotic prophase chromosome cores, and a M(r) 125,000 Syn1 protein is present between homologue cores where they are synapsed and form the synaptonemal complex (SC). Immunolocalization of these proteins during meiosis suggests possible homo- and heterotypic interactions between the two as well as possible interactions with yet unrecognized proteins. We used the two-hybrid system in the yeast Saccharomyces cerevisiae to detect possible protein-protein associations. Segments of hamsters Cor1 and Syn1 proteins were tested in various combinations for homo- and heterotypic interactions. In the cause of Cor1, homotypic interactions involve regions capable of coiled-coil formation, observation confirmed by in vitro affinity coprecipitation experiments. The two-hybrid assay detects no interaction of Cor1 protein with central and C-terminal fragments of Syn1 protein and no homotypic interactions involving these fragments of Syn1. Hamster Cor1 and Syn1 proteins both associate with the human ubiquitin-conjugation enzyme Hsubc9 as well as with the hamster Ubc9 homologue. The interactions between SC proteins and the Ubc9 protein may be significant for SC disassembly, which coincides with the repulsion of homologs by late prophase I, and also for the termination of sister centromere cohesiveness at anaphase II.

Author List

Tarsounas M, Pearlman RE, Gasser PJ, Park MS, Moens PB

Author

Paul Gasser BS,MS,PhD Assistant Professor in the Biomedical Sciences department at Marquette University

MESH terms used to index this publication - Major topics in bold

Animals
Cricetinae
DNA Primers
DNA-Binding Proteins
Humans
Ligases
Molecular Sequence Data
Nuclear Proteins
Polymerase Chain Reaction
Recombinant Fusion Proteins
Synaptonemal Complex
Ubiquitin-Conjugating Enzymes