Faculty Collaboration Database

Cytokeratin expression in seminoma of the human testis. Am J Clin Pathol 2000 Apr;113(4):583-8

Date

04/13/2000

Pubmed ID

10761461

DOI

10.1309/5fu2-8yq9-q12r-y0ku

Scopus ID

2-s2.0-0034093274 (requires institutional sign-in at Scopus site)   93 Citations

Abstract

We studied cytokeratin (CK) expression immunohistochemically in 64 seminomas using a panel of commercially available antikeratin antibodies and tested for association of CK expression with patient age, tumor size, stage, and outcome. Seventeen embryonal carcinomas were compared with seminoma. CK7, CAM 5.2, AEI/AEIII, and wide-spectrum screening keratin (WSK) were positive in 41%, 30%, 36%, and 36% of the seminomas, respectively. CK20 and high-molecular-weight keratin (HMWK) were negative in all cases. CD30, placental alkaline phosphatase (PLAP), and epithelial membrane antigen (EMA) were positive in 6%, 100%, and 2% of cases, respectively. There were no differences in patient age, stage, tumor size, or outcome between CK-positive and CK-negative seminomas. CK7, CAM 5.2, AEI/AEIII, and WSK were positive in 100%, 88%, 94%, and 88% of embryonal carcinomas, respectively. CK20 and HMWK were negative in all cases. CD30, EMA, and PLAP were positive in 100%, 12%, and 76%, respectively. CKs are present in seminoma, and their presence is not associated with a difference in patient age, stage, or outcome. In cases such as small needle biopsy specimens, CK and CD30 stains may be useful in separating seminoma from embryonal carcinoma.

Author List

Cheville JC, Rao S, Iczkowski KA, Lohse CM, Pankratz VS

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Alkaline Phosphatase
Carcinoma, Embryonal
Germinoma
Humans
Immunohistochemistry
Keratins
Male
Middle Aged
Mucin-1
Neoplasm Staging
Seminoma
Testicular Neoplasms