Medical College of Wisconsin
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Induction of human cholesterol 7alpha-hydroxylase in HepG2 cells by 2,4,6-trihydroxyacetophenone. Eur J Pharmacol 2005 May 16;515(1-3):43-6

Date

05/18/2005

Pubmed ID

15896733

DOI

10.1016/j.ejphar.2005.03.039

Scopus ID

2-s2.0-20344362658 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

In animal the plasma cholesterol-lowering activity of 2,4,6-trihydroxyacetophenone (THA) is due to enhanced cholesterol 7alpha-hydroxylase (CYP7A1) activity. We have examined the effect of THA on CYP7A1 activity and mRNA level in HepG2 cells. THA stimulated CYP7A1 activity in a concentration- and time-dependent manner. After exposure for 24 h, 1 muM THA induced CYP7A1 activity 160+/-8% and mRNA level 166+/-21% (mean+/-S.E.M.) of control. Moreover THA antagonized the inhibitory regulation of chenodeoxycholic acid on CYP7A1 mRNA expression. These results indicated that THA increases CYP7A1 activity in human HepG2 cells by stimulating mRNA transcription.

Author List

Charoenteeraboon J, Nithipatikom K, Campbell WB, Piyachaturawat P, Wilairat P, Rongnoparut P

Author

William B. Campbell PhD Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Acetophenones
Cell Line, Tumor
Cholesterol 7-alpha-Hydroxylase
Dose-Response Relationship, Drug
Gene Expression Regulation, Enzymologic
Humans
Microsomes
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction