Separation and quantitation of phospholipid hydroperoxide families using high-performance liquid chromatography with mercury cathode electrochemical detection. Anal Biochem 2005 Aug 01;343(1):136-42
Date
06/28/2005Pubmed ID
15979556DOI
10.1016/j.ab.2005.04.005Scopus ID
2-s2.0-22144489919 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
High-performance liquid chromatography with mercury cathode electrochemical detection (HPLC-EC(Hg)) was used to separate and quantify various phospholipid hydroperoxide (PLOOH) families. Under the conditions used, baseline separation of four major biologically relevant PLOOH classes was achieved. Responsiveness was linear up to at least 1 nmol of PLOOH with a detection limit in the subpicomolar range (0.1-0.5 pmol). Applying this method to photodynamically stressed murine leukemia cells and mitochondria isolated from these cells, we identified and quantified PLOOHs derived from phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and cardiolipin. In terms of high sensitivity, specificity, and reliability, HPLC-EC(Hg) has a clear advantage over all other existing techniques for determining PLOOHs in complex biological systems.
Author List
Korytowski W, Niziolek M, Girotti AWAuthor
Albert W. Girotti PhD Adjunct Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCattle
Cell Line
Chromatography, High Pressure Liquid
Electrochemistry
Electrodes
Glycerophospholipids
Lipid Peroxides
Mercury
Mice
Mitochondria
