Quantitation of spinal cord demyelination, remyelination, atrophy, and axonal loss in a model of progressive neurologic injury. J Neurosci Res 1999 Nov 15;58(4):492-504
Date
10/26/1999Pubmed ID
10533042Pubmed Central ID
PMC5451093DOI
10.1002/(sici)1097-4547(19991115)58:4<492::aid-jnr3>3.0.co;2-pScopus ID
2-s2.0-0033571378 (requires institutional sign-in at Scopus site) 76 CitationsAbstract
Spinal cord pathology, such as demyelination and axonal loss, is a common feature in multiple models of central nervous system (CNS) injury and disease. Development of methods to quantify spinal cord pathology objectively would aid studies designed to establish mechanisms of damage, correlate pathology with neurologic function, and assess therapeutic interventions. In this study, we describe sensitive methods to objectively quantify spinal cord demyelination, remyelination, atrophy, and axonal loss following the initiation of a progressive inflammatory demyelinating disease with Theiler's murine encephalomyelitis virus (TMEV). Spinal cord demyelination, remyelination, and atrophy were quantified from representative 1-microm-thick cross sections embedded in Araldite plastic using interactive image analysis. In addition, this study demonstrates novel, automated methodology to quantify axonal loss from areas of normal-appearing white matter, as a measure of secondary axonal injury following demyelination. These morphologic methods, which are applicable to various models of CNS injury, provide an innovative way to assess the benefits of therapeutic agents, to determine mechanisms of spinal cord damage, or to establish a correlation with sensitive measures of neurologic function. J. Neurosci Res 58:492-504.
Author List
McGavern DB, Murray PD, Rodriguez MAuthor
Paul D. Harker-Murray PhD, MD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAtrophy
Axons
Cell Count
Central Nervous System
Demyelinating Diseases
Mice
Mice, Inbred Strains
Mice, Knockout
Myelin Sheath
Neurons
Poliomyelitis
Spinal Cord
Theilovirus
beta 2-Microglobulin
