Faculty Collaboration Database

Genome-wide scanning with SSLPs in the rat. Methods Mol Med 2005;108:131-8

Date

07/21/2005

Pubmed ID

16028680

DOI

10.1385/1-59259-850-1:131

Scopus ID

2-s2.0-24644495136 (requires institutional sign-in at Scopus site)   29 Citations

Abstract

Genome-wide linkage analysis is a powerful tool for the identification of genes underlying single gene and complex genetic disorders. The most commonly used technique for performing genome wide scans for genetic studies in the rat is by analysis of simple sequence length polymorphism (SSLPs) or microsatellite markers. A sensitive and flexible method for high-throughput genotyping is described. Addition of an M13 tail to the SSLP primer eliminates the need for direct conjugation of the fluorescent dye to the primers, allowing for any combination of primer and fluorophor, and therefore for easy multiplexing of primers in the same reaction. With the use of three different dyes, it is possible to run more than five hundred genotypes in each run of the automatic sequencer. Automation in the fluorescent detection system and data tracking software for processing genotypes, contributes to the ability to genotype large number of samples rapidly and accurately.

Author List

Moreno C, Kennedy K, Andrae JW, Jacob HJ

MESH terms used to index this publication - Major topics in bold

Animals
Base Sequence
DNA Primers
Genetic Markers
Genome
Microsatellite Repeats
Polymerase Chain Reaction
Polymorphism, Genetic
Quantitative Trait Loci
Rats
Sensitivity and Specificity