Blood platelet kinetics and platelet transfusion. J Clin Invest 2013 Nov;123(11):4564-5
Date
11/02/2013Pubmed ID
24177466Pubmed Central ID
PMC3809797DOI
10.1172/JCI70335Scopus ID
2-s2.0-84887458879 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
The discovery of citrate anticoagulant in the 1920s and the development of plastic packs for blood collection in the 1960s laid the groundwork for platelet transfusion therapy on a scale not previously possible. A major limitation, however, was the finding that platelet concentrates prepared from blood anticoagulated with citrate were unsuitable for transfusion because of platelet clumping. We found that this could be prevented by simply reducing the pH of platelet-rich plasma to about 6.5 prior to centrifugation. We used this approach to characterize platelet kinetics and sites of platelet sequestration in normal and pathologic states and to define the influence of variables such as anticoagulant and ABO incompatibility on post-transfusion platelet recovery. The "acidification" approach enabled much wider use of platelet transfusion therapy until alternative means of producing concentrates suitable for transfusion became available.
Author List
Aster RHAuthor
Richard Aster MD Emeritus Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnticoagulantsBlood Platelets
Blood Specimen Collection
Citric Acid
Glucose
History, 20th Century
History, 21st Century
Humans
Hydrogen-Ion Concentration
Platelet Aggregation
Platelet Transfusion
Thrombocytopenia
