The fibrinolysis inhibitor alpha2-antiplasmin in the human cornea. Curr Eye Res 2005 Dec;30(12):1097-103
Date
12/16/2005Pubmed ID
16354623DOI
10.1080/02713680500403046Scopus ID
2-s2.0-29144449414 (requires institutional sign-in at Scopus site) 4 CitationsAbstract
PURPOSE: To determine whether the cornea contains and expresses, at the gene level, the major plasmin inhibitor alpha2-antiplasmin.
METHODS: Corneal sections were immunostained for alpha2-antiplasmin. Extracts of human corneal stroma, epithelium, and endothelium were subjected to immunodot blot and Western blot analysis. Total RNA and alpha2-antiplasmin specific primers were used for RT-PCR. The cDNA was sequenced.
RESULTS: Alpha2-antiplasmin was observed in all three corneal layers by immunolocalization and Western blots. The major alpha2-antiplasmin form observed in most extracts was the 70-kDa form. Total alpha2-antiplasmin was present at 0.119 +/- 0.014 microg/epithelium (n = 10) and 1.45 +/- 0.47 microg/stroma (n = 10). Alpha2-antiplasmin mRNA was detected in epithelial and stromal extracts and cultured human corneal stromal cells. The sequences of the PCR products were identical to that for human alpha2-antiplasmin.
CONCLUSIONS: Alpha2-antiplasmin and its mRNA are present in the cornea and may serve to regulate corneal plasmin activity.
Author List
Ayala A, Warejecka DJ, Vaughan KA, Twining SS, Yue BYAuthor
Sally S. Twining PhD Assistant Dean, Professor in the Biochemistry department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Aged
Aged, 80 and over
Antifibrinolytic Agents
Base Sequence
Blotting, Western
Corneal Stroma
Endothelium, Corneal
Epithelium, Corneal
Humans
Immunoblotting
Immunoenzyme Techniques
Middle Aged
Molecular Sequence Data
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
alpha-2-Antiplasmin
