Characterization of CD 200-receptor expression in the murine epidermis. J Invest Dermatol 2005 Dec;125(6):1130-8
Date
12/16/2005Pubmed ID
16354182DOI
10.1111/j.0022-202X.2005.23948.xScopus ID
2-s2.0-32644457432 (requires institutional sign-in at Scopus site) 26 CitationsAbstract
CD 200 is a widely expressed transmembrane glycoprotein that transmits an inhibitory signal after ligation of the structurally homologous CD 200-receptor-1 (CD 200 R1). Recently, we showed that CD 200 is expressed on keratinocytes and plays a role in protecting hair follicles from autoimmune attack. Here, we report the characterization of cell surface and mRNA expression of CD 200 R1 by cells of the murine epidermis. In addition, we report mRNA expression for other members of the CD 200 R-family (R2-R4) by quantitative real-time RT-PCR. Variable levels of CD 200 R1, R2, R3, and R4 mRNA were detected in bulk epidermal cell suspensions. Freshly isolated Langerhans cells (LC) preferentially expressed CD 200 R1. Consistent with an inhibitory role for CD 200:CD 200 R1 interaction, LC obtained from mice deficient in CD 200 (CD 200(-/-)) were in a heightened state of activation as compared with wild-type (CD 200(+/+)) cells. Freshly isolated dendritic epidermal T cells (DETC) expressed low levels of CD 200 R1, R2, and R3 mRNA, but they preferentially increased cell surface and mRNA expression of CD 200 R1 upon activation in vitro. In functional assays using sub-optimal CD3 signaling, immobilized CD 200 inhibited DETC proliferation and cytokine secretion. Collectively, these results suggest that CD 200:CD 200 R interactions may play a role in regulating both LC and DETC in cutaneous immune reactions.
Author List
Rosenblum MD, Woodliff JE, Madsen NA, McOlash LJ, Keller MR, Truitt RLMESH terms used to index this publication - Major topics in bold
AnimalsAntigens, Surface
Cell Line
Dendritic Cells
Ear
Epidermis
Flow Cytometry
Membrane Glycoproteins
Mice
Mice, Inbred C57BL
Mice, Knockout
Orexin Receptors
RNA, Messenger
Receptors, Antigen, T-Cell, gamma-delta
Receptors, Cell Surface
Recombinant Fusion Proteins
Reverse Transcriptase Polymerase Chain Reaction
