Graft versus leukemia. VIII. Selective reduction in antihost reactivity without loss of antileukemic reactivity by treatment of donor mice with lipopolysaccharide. Exp Hematol 1978 May;6(5):488-98
Date
05/01/1978Pubmed ID
26584Scopus ID
2-s2.0-0017898443 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Spleen and lymph node cells from DBA/2 (H-2d) donor mice treated with multiple injections of bacterial lipopolysaccharide (LPS) were tested in vivo for reactivity against normal tissues of host AKR (H-2k) mice against an AKR long-passage, acute lymphoblastic leukemia (BW5147). LPS treatment of donor mice resulted in a reduction in graft-versus-host (GVH) reactivity without loss of graft-versus-leukemia (GVL) reactivity. Immunocompetent cells from LPS treated DBA/2 donors were effective when used for adoptive immunotherapy (in combination with chemoradiotherapy) of BW5147 leukemia. GVH associated mortality decreased as the dose of spleen cells from LPS treated histoincompatible donors was increased as much as four times the number necessary to eliminate leukemia. The mechanism by which LPS reduced GVH reactivity without eliminating GVL reactivity is unclear; however, it does not appear to be the result of a dilution in the number of GVH reactive cells by nonlymphoid elements in the donor spleen nor of the adjuvant effects of LPS on resistance to bacterial infections.
Author List
Truitt RL, Rose WC, Rimm AA, Bortin MMAuthor
Robert L. Truitt PhD Emeritus Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCyclophosphamide
Graft vs Host Reaction
Leukemia, Experimental
Leukemia, Lymphoid
Lipopolysaccharides
Lymphocytes
Mice
Mice, Inbred AKR
Mice, Inbred C57BL
Mice, Inbred DBA
Spleen
Transplantation, Homologous
