Enhanced large conductance K+ channel activity contributes to the impaired myogenic response in the cerebral vasculature of Fawn Hooded Hypertensive rats. Am J Physiol Heart Circ Physiol 2014 Apr 01;306(7):H989-H1000
Date
01/28/2014Pubmed ID
24464756Pubmed Central ID
PMC3962634DOI
10.1152/ajpheart.00636.2013Scopus ID
2-s2.0-84900531560 (requires institutional sign-in at Scopus site) 24 CitationsAbstract
Recent studies have indicated that the myogenic response (MR) in cerebral arteries is impaired in Fawn Hooded Hypertensive (FHH) rats and that transfer of a 2.4 megabase pair region of chromosome 1 (RNO1) containing 15 genes from the Brown Norway rat into the FHH genetic background restores MR in a FHH.1(BN) congenic strain. However, the mechanisms involved remain to be determined. The present study examined the role of the large conductance calcium-activated potassium (BK) channel in impairing the MR in FHH rats. Whole-cell patch-clamp studies of cerebral vascular smooth muscle cells (VSMCs) revealed that iberiotoxin (IBTX; BK inhibitor)-sensitive outward potassium (K+) channel current densities are four- to fivefold greater in FHH than in FHH.1(BN) congenic strain. Inside-out patches indicated that the BK channel open probability (NPo) is 10-fold higher and IBTX reduced NPo to a greater extent in VSMCs isolated from FHH than in FHH.1(BN) rats. Voltage sensitivity of the BK channel is enhanced in FHH as compared with FHH.1(BN) rats. The frequency and amplitude of spontaneous transient outward currents are significantly greater in VSMCs isolated from FHH than in FHH.1(BN) rats. However, the expression of the BK-α and -β-subunit proteins in cerebral vessels as determined by Western blot is similar between the two groups. Middle cerebral arteries (MCAs) isolated from FHH rats exhibited an impaired MR, and administration of IBTX restored this response. These results indicate that there is a gene on RNO1 that impairs MR in the MCAs of FHH rats by enhancing BK channel activity.
Author List
Pabbidi MR, Mazur O, Fan F, Farley JM, Gebremedhin D, Harder DR, Roman RJMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Congenic
Calcium Signaling
Cerebrovascular Circulation
Disease Models, Animal
Hypertension
Ion Channel Gating
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
Large-Conductance Calcium-Activated Potassium Channel beta Subunits
Large-Conductance Calcium-Activated Potassium Channels
Male
Membrane Potentials
Middle Cerebral Artery
Muscle, Smooth, Vascular
Myocytes, Smooth Muscle
Patch-Clamp Techniques
Potassium Channel Blockers
Rats
Rats, Inbred BN
Vasoconstriction
