Faculty Collaboration Database

Kinetic analysis of human IL-2 activated cytotoxic cells. Immunopharmacol Immunotoxicol 1991;13(1-2):147-68

Date

01/01/1991

Pubmed ID

1770217

DOI

10.3109/08923979109019697

Scopus ID

2-s2.0-0025767215 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

Kinetic analysis was used to define lytic events in peripheral blood mononuclear cells (PBMC) activated in lymphokine conditioned medium (LCM) and recombinant interleukin-2 (rIL-2). This analysis provided quantitative information on the functional properties of these lymphokine-activated killer (LAK) cells against NK-resistant and NK-sensitive tumor cell lines. The maximum rate of target cell lysis (Vmax) and Km (target cell number resulting in 1/2 Vmax) were determined. IL-2 activated effector cells that bound to target cells also lysed them (i.e., non-lytic bystander lymphocytes did not influence the determination of kinetic parameters) in contrast to lysis mediated by unactivated NK cells. The extent of LAK cell binding to tumor target cells was dependent upon the tumor type. LAK cell frequency determinations were calculated where Km approximated the concentration of LAK cells that were capable of killing a particular target. LAK cells generated in rIL-2 were lytically more efficient than those activated in LCM, and T-depletion resulted in a LAK population with the highest maximum rate of lysis. The use of kinetic analysis to evaluate LAK cell frequencies and quantitate lytic events will be useful in determining the effects of drugs, biological response modifiers and disease states on LAK cell function.

Author List

LeFever AV, Piaskowski VD, Casper JT, Truitt RL

Authors

James Casper MD Emeritus Professor in the Pediatrics department at Medical College of Wisconsin
Robert L. Truitt PhD Emeritus Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Cytotoxicity, Immunologic
Humans
In Vitro Techniques
Interleukin-2
Killer Cells, Lymphokine-Activated
Kinetics
Lymphocyte Depletion
T-Lymphocytes
Tumor Cells, Cultured