Localization of bFGF-like proteins as punctate inclusions in the preseptation myocardium of the chicken embryo. Dev Biol 1991 Jul;146(1):139-47
Date
07/01/1991Pubmed ID
1647988DOI
10.1016/0012-1606(91)90454-bScopus ID
2-s2.0-0025812005 (requires institutional sign-in at Scopus site) 72 CitationsAbstract
Immunocytochemistry has been employed to map the appearance of bFGF-like proteins in precardiac and preseptation myocardial cells between stages 6 and 15 of chicken embryogenesis. Stage 6 embryos exhibited no staining, with the exception of a subtle signal in endoderm cells. At subsequent stages, staining was observed only in cells of the developing myocardium, first appearing at the time of heart tube fusion (stage 9+) as punctate cytoplasmic aggregates. While the expression of bFGF-like antigen was temporally similar to that of myosin heavy chain, their staining patterns differed in that bFGF-like proteins were nonsarcomeric and did not extend into the inflow or outflow tracts. Western blotting of heparin agarose affinity-isolated proteins from stage 15 hearts revealed an antigen migrating at approximately 19 kDa. In contrast with the unique localization of bFGF-like proteins in myocardial cells, FGF receptor (FGFR) staining was widely distributed in the embryo; however, concentrated deposits of FGFR were detected in endothelial and myocardial cells, which diminished in the myocardium but not in the endothelium by stage 15. These results suggest that FGF-like proteins may have autocrine and/or paracrine functions during early cardiac morphogenesis.
Author List
Parlow MH, Bolender DL, Kokan-Moore NP, Lough JMESH terms used to index this publication - Major topics in bold
AnimalsChick Embryo
Endothelium, Vascular
Fibroblast Growth Factor 2
Heart
Inclusion Bodies
Molecular Weight
Myocardium
Myosins
