Faculty Collaboration Database

Absence of an interaction between the Rf-1 and Rf-5 QTLs influencing susceptibility to renal damage in rats. Nephron Exp Nephrol 2006;104(3):e96-e102

Date

07/14/2006

Pubmed ID

16837819

DOI

10.1159/000094548

Scopus ID

2-s2.0-33750038004 (requires institutional sign-in at Scopus site)   7 Citations

Abstract

BACKGROUND: Previous studies showed that combining the Rf-1 and Rf-3 or Rf-4 QTLs of FHH induced synergistic interactions markedly enhancing renal susceptibility. The present study aimed to determine the presence of such interaction between the Rf-1 and Rf-5 QTLs.

METHODS: Renal damage susceptibility was assessed in Rf-1B, Rf-1B+5, Rf-1B+4 congenics and ACI control rats in four situations: two-kidney control (2K), unilateral nephrectomy (UNX), L-NAME-induced hypertension (2K+L-NAME) and UNX+L-NAME. Albuminuria (UAV) and systolic blood pressure (SBP) were measured during 18 weeks of follow-up. In separate experiments, renal autoregulation was assessed in 2K rats.

RESULTS: In all four situations, Rf-1B+4 rats developed more severe UAV than ACI, Rf-1B and Rf-1B+5. There were no significant differences in UAV between Rf-1B and Rf-1B+5 rats. In the 2K and UNX situation no differences in SBP were noted between all four strains. With 2K+L-NAME and UNX+L-NAME treatment, SBP in double congenics was higher than that of ACI and Rf-1B rats. Renal autoregulation was similarly impaired in all three congenic strains.

CONCLUSION: We conclude that the Rf-5 region, alone or in the presence of Rf-1B, does not affect the development of renal damage. We cannot substantiate that the Rf-5 region contains genes influencing renal damage susceptibility.

Author List

van Dijk SJ, Specht PA, Lazar J, Jacob HJ, Provoost AP

MESH terms used to index this publication - Major topics in bold

Albuminuria
Animals
Animals, Congenic
Blood Pressure
Enzyme Inhibitors
Epistasis, Genetic
Genetic Predisposition to Disease
Glomerulosclerosis, Focal Segmental
Homeostasis
Hypertension
NG-Nitroarginine Methyl Ester
Nephrectomy
Quantitative Trait Loci
Rats
Rats, Inbred Strains
Renal Circulation
Renal Insufficiency
Survival Analysis