Gastro-intestinal exposure to latex antigens induce allergic responses in mice. Int Arch Allergy Immunol 2006;141(2):158-67
Date
08/11/2006Pubmed ID
16899983DOI
10.1159/000094894Scopus ID
2-s2.0-33748799680 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
BACKGROUND: Natural rubber latex (NRL) has emerged as a major cause of respiratory allergy among specific exposed groups of individuals. Since latex allergens are dispersed in the environment it is conceivable that latex proteins are both inhaled and ingested. The mechanism of latex allergy and the immune responses following reexposure of latex allergens by the intranasal route was studied in a murine model of latex allergy developed by intragastric sensitization with NRL.
METHODS: BALB/c mice were sensitized intragastrically ('ig'), intranasally ('in') or 'ig' followed by 'in' challenge with NRL allergens. The cellular and humoral immune responses, lung function and histological changes were determined.
RESULTS: Peripheral blood eosinophilia was observed in the 'ig' and 'ig/in'-NRL-sensitized animals in comparison to normal controls (p < 0.05). The 'ig' group showed a marked increase over control mice in serum total IgE, NRL-specific IgG and IgG subclasses (p < 0.05). Increased levels of IL-4, IL-5, IL-10, and IL-13 were detected in 'ig'-NRL-sensitized mice. Intranasal exposure with NRL after 'ig' sensitization further enhanced the cytokine levels. A tendency towards enhanced stimulation was determined in 'ig'-sensitized mice; a significant difference was shown in the 'ig/in'-group (p < 0.05). Increased airway hyperreactivity was found in 'ig'-NRL-sensitized-mice (15.1 +/- 2.5 vs. 8.9 +/- 1.7 cm H2O x ml(-1) x s, p < 0.05). Mucus secretion from jejunal epithelium and eosinophilic infiltration into the jejunal lamina propria were observed in the 'ig'-NRL-sensitized-mice.
CONCLUSIONS: The results demonstrate that intragastric NRL sensitization did not induce specific tolerance, and additional intranasal exposure with latex allergens resulted in systemic allergic manifestations in the murine model.
Author List
Barrios CS, Kurup VP, Rickaby DA, Henderson JD Jr, Fink JN, Kelly KJMESH terms used to index this publication - Major topics in bold
Administration, IntranasalAnimals
Bronchial Hyperreactivity
Bronchial Provocation Tests
Chemokine CCL5
Disease Models, Animal
Drug Administration Routes
Eosinophilia
Female
Immune Tolerance
Immunoglobulin E
Immunoglobulin G
Interleukin-10
Interleukin-13
Interleukin-4
Interleukin-5
Intestines
Latex
Latex Hypersensitivity
Lung
Lymphocyte Activation
Mice
Stomach
T-Lymphocytes
