N-glycoprotein surfaceomes of four developmentally distinct mouse cell types. Proteomics Clin Appl 2014 Aug;8(7-8):603-9
Date
06/13/2014Pubmed ID
24920426Pubmed Central ID
PMC4151168DOI
10.1002/prca.201400021Scopus ID
2-s2.0-84906055064 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
PURPOSE: Detailed knowledge of cell surface proteins present during early embryonic development remains limited for most cell lineages. Due to the relevance of cell surface proteins in their functional roles controlling cell signaling and their utility as accessible, nongenetic markers for cell identification and sorting, the goal of this study was to provide new information regarding the cell surface proteins present during early mouse embryonic development.
EXPERIMENTAL DESIGN: Using the cell surface capture technology, the cell surface N-glycoproteomes of three cell lines and one in vitro differentiated cell type representing distinct cell fates and stages in mouse embryogenesis were assessed.
RESULTS: Altogether, more than 600 cell surface N-glycoproteins were identified represented by >5500 N-glycopeptides.
CONCLUSIONS AND CLINICAL RELEVANCE: The development of new, informative cell surface markers for the reliable identification and isolation of functionally defined subsets of cells from early developmental stages will advance the use of stem cell technologies for mechanistic developmental studies, including disease modeling and drug discovery.
Author List
Kropp EM, Bhattacharya S, Waas M, Chuppa SL, Hadjantonakis AK, Boheler KR, Gundry RLMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Cell Line
Embryonic Development
Glycoproteins
Mice
Molecular Sequence Data
Proteomics
