Faculty Collaboration Database

Analysis of metabolites in plasma reveals distinct metabolic features between Dahl salt-sensitive rats and consomic SS.13(BN) rats. Biochem Biophys Res Commun 2014 Jul 18;450(1):863-9

Date

06/28/2014

Pubmed ID

24971531

Pubmed Central ID

PMC4423742

DOI

10.1016/j.bbrc.2014.06.089

Scopus ID

2-s2.0-84904759537 (requires institutional sign-in at Scopus site)   40 Citations

Abstract

Salt-sensitive hypertension is a major risk factor for cardiovascular disorders. Our previous proteomic study revealed substantial differences in several proteins between Dahl salt-sensitive (SS) rats and salt-insensitive consomic SS.13(BN) rats. Subsequent experiments indicated a role of fumarase insufficiency in the development of hypertension in SS rats. In the present study, a global metabolic profiling study was performed using gas chromatography/mass spectrometry (GC/MS) in plasma of SS rats (n=9) and SS.13(BN) rats (n=8) on 0.4% NaCl diet, designed to gain further insights into the relationship between alterations in cellular intermediary metabolism and predisposition to hypertension. Principal component analysis of the data sets revealed a clear clustering and separation of metabolic profiles between SS rats and SS.13(BN) rats. 23 differential metabolites were identified (P<0.05). Higher levels of five TCA cycle metabolites, fumarate, cis-aconitate, isocitrate, citrate and succinate, were observed in SS rats. Pyruvate, which connects TCA cycle and glycolysis, was also increased in SS rats. Moreover, lower activity levels of fumarase, aconitase, α-ketoglutarate dehydrogenase and succinyl-CoA synthetase were detected in the heart, liver or skeletal muscles of SS rats. The distinct metabolic features in SS and SS.13(BN) rats indicate abnormalities of TCA cycle in SS rats, which may play a role in predisposing SS rats to developing salt-sensitive hypertension.

Author List

Wang L, Hou E, Wang Z, Sun N, He L, Chen L, Liang M, Tian Z

MESH terms used to index this publication - Major topics in bold

Animals
Blood Pressure
Blood Proteins
Genetic Predisposition to Disease
Hypertension
Inbreeding
Proteome
Rats
Rats, Inbred BN
Rats, Inbred Dahl
Sodium Chloride, Dietary