Faculty Collaboration Database

Interleukin-10 attenuates the response to vascular injury. J Surg Res 2004 Oct;121(2):206-13

Date

10/27/2004

Pubmed ID

15501460

DOI

10.1016/j.jss.2004.03.025

Scopus ID

2-s2.0-6344258590 (requires institutional sign-in at Scopus site)   41 Citations

Abstract

BACKGROUND: The inflammatory response to vascular injury is characterized by expression of cytokines, growth factors, and chemokines that conspire to promote vessel remodeling and intimal hyperplasia (IH). Interleukin-10 (IL-10) is a multifunctional cytokine that has several anti-inflammatory properties in vitro. Few studies have evaluated the effects of IL-10 in experimental atherosclerosis. The purpose of the present study was to determine the influence of IL-10 on vascular inflammation and IH following mechanical injury.

METHODS: Wire carotid injury was performed in wild-type (WT) mice with and without IL-10 treatment. Immunohistochemistry, PCR, and ELISA assays were used to examine vessel production of basic fibroblast growth factor (bFGF), monocyte chemotactic protein-1 (MCP-1), and nuclear factor kappa B (NFkappaB). Vessels were morphometrically analyzed for IH.

RESULTS: Carotid injury induced early expression of MCP-1 and bFGF that was abrogated in mice treated with IL-10. Similarly, injury-induced expression of NFkappaB message and protein was attenuated in mice receiving exogenous IL-10. Compared to untreated mice, IL-10 markedly decreased levels of IH. Interestingly, carotid injury in IL-10-deficient mice resulted in an augmented IH response compared to injured WT mice.

CONCLUSIONS: In an in vivo model of direct vascular injury, IL-10 decreased expression of the pro-inflammatory transcription factor, NFkappaB, and the mitogenic chemokine and growth factor, MCP-1 and bFGF, respectively. These observations were associated with IL-10-induced attenuation of IH. Furthermore, endogenous IL-10 appeared to suppress the injury response. In conclusion, exogenously delivered IL-10 may represent a clinically relevant anti-inflammatory strategy for post-injury intimal hyperplasia.

Author List

Zimmerman MA, Reznikov LL, Raeburn CD, Selzman CH

MESH terms used to index this publication - Major topics in bold

Animals
Carotid Arteries
Carotid Artery Injuries
Chemokines
Growth Substances
Hyperplasia
Immunohistochemistry
Interleukin-10
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
NF-kappa B
Reverse Transcriptase Polymerase Chain Reaction
Tunica Intima
Vasculitis
Wounds, Nonpenetrating