Plasma membrane properties regulating the sensitivity of leukemia, lymphoma, and solid tumor cells to merocyanine 540-sensitized photoirradiation. Exp Hematol 1991 Sep;19(8):785-8
Date
09/01/1991Pubmed ID
1868893Scopus ID
2-s2.0-0025832172 (requires institutional sign-in at Scopus site) 20 CitationsAbstract
Merocyanine 540 (MC 540) is a photosensitizing dye that has been used in a phase I clinical trial for the purging of leukemia and lymphoma cells from autologous bone marrow grafts. In this paper we examine the role of plasma membrane negative charge, plasma membrane fluidity, and plasma membrane hydrophobicity in the regulation of a cell's susceptibility to MC 540-sensitized photoirradiation. Among solid tumor cells, we found an inverse correlation between surface electronegativity, affinity for dye molecules, and susceptibility to MC 540-sensitized photoinactivation. That is, the least electronegative cells bound the highest amount of dye and were the most susceptible to dye-sensitized photoirradiation. By contrast, no such correlations were found among leukemia/lymphoma cells. This suggested that dye binding and susceptibility to MC 540-mediated photodynamic damages are regulated differently in hematopoietic/lymphopoietic and solid tumor cells.
Author List
Smith OM, Gaffney DK, Anderson MS, McOlash L, Schober SL, Sieber FAuthor
Fritz Sieber PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Bone MarrowBone Marrow Cells
Cell Membrane
Cell Separation
Humans
Leukemia
Light
Lymphoma
Membrane Fluidity
Neoplasms
Neuraminidase
Pyrimidinones
Radiation-Sensitizing Agents
Solubility
Surface Properties
Trypsin
Tumor Cells, Cultured
