Lymph is not a plasma ultrafiltrate: a proteomic analysis of injured patients. Shock 2014 Dec;42(6):485-498
Date
09/23/2014Pubmed ID
25243428Pubmed Central ID
PMC4236236DOI
10.1097/SHK.0000000000000249Scopus ID
2-s2.0-84930042518 (requires institutional sign-in at Scopus site) 39 CitationsAbstract
Studies on animal models have documented a role for the water-soluble protein fraction of mesenteric lymph as a conduit from hemorrhagic shock to acute lung injury and postinjury multiple organ failure. We hypothesize that mesenteric lymph is not an ultrafiltrate of plasma and contains specific protein mediators that may predispose patients to acute lung injury/multiple organ failure. Mesenteric lymph and plasma were collected from critically ill or injured patients and from nine patients with lymphatic injuries, during semielective spine reconstruction, or immediately before organ donation. Proteomic analyses were performed through immunoaffinity depletion of the 14 most abundant plasma proteins and 1D gel electrophoresis followed by liquid chromatography coupled online with mass spectrometry analyses. Overall, 548 proteins were identified in the patients undergoing semielective surgery, of which 155 were uniquely present in the lymph. In addition, the postshock plasma proteome was characterized by peculiar features, suggesting that only a partial overlap exists between the plasma and mesenteric lymph from trauma patients. Differential proteins between the matched plasma and mesenteric lymph from trauma patients could be related to coagulopathy and hypercoagulability, cell lysis, proinflammatory responses and immune system activation, extracellular matrix remodeling, lymph-specific immunomodulation and vascular hypoactivity/neoangiogenesis, and energy/redox metabolic adaptation to trauma. In conclusion, the proteome of mesenteric lymph is biologically different (in qualitative and quantitative terms) than that of a mere plasma ultrafiltrate.
Author List
Dzieciatkowska M, D'Alessandro A, Moore EE, Wohlauer M, Banerjee A, Silliman CC, Hansen KCMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Blood Coagulation
Blood Proteins
Chromatography, Liquid
Cluster Analysis
Extracellular Matrix Proteins
Female
Humans
Inflammation
Lymph
Male
Mass Spectrometry
Mesentery
Middle Aged
Oxidation-Reduction
Oxidative Stress
Proteome
Proteomics
Tandem Mass Spectrometry
Wounds and Injuries
Young Adult
