Faculty Collaboration Database

Effect of high-salt diet on vascular relaxation and oxidative stress in mesenteric resistance arteries. J Vasc Res 2007;44(5):382-90

Date

05/19/2007

Pubmed ID

17510561

DOI

10.1159/000102955

Scopus ID

2-s2.0-34548171957 (requires institutional sign-in at Scopus site)   101 Citations

Abstract

This study tested the hypothesis that superoxide levels are elevated in isolated mesenteric resistance arteries (100-300 microm) from rats fed a short-term high-salt (HS) diet (4% NaCl for 3 days) compared to controls fed a low-salt (LS) diet (0.4% NaCl). Vascular relaxation induced by the superoxide dismutase mimetic tempol (4-hydroxytetramethylpiperidine-1-oxyl), the NADPH oxidase inhibitor apocynin and the xanthine/xanthine oxidase inhibitor oxypurinol was significantly larger in mesenteric arteries from animals fed HS diet compared to arteries from animals fed LS diet. Basal superoxide levels assessed via dihydroethidine (DHE) fluorescence were significantly elevated in arteries from rats fed HS diet, and were reduced by tempol, apocynin and oxypurinol, but not by L-NAME. Basal and methacholine-induced NO production (assessed by DAF-2T fluorescence) was significantly reduced in arteries from rats fed HS diet versus arteries from rats on LS diet. Impaired methacholine-induced NO release and vascular relaxation were restored by tempol and apocynin, but not by oxypurinol. These data suggest that the elevated production of superoxide by NADPH oxidase and xanthine/xanthine oxidase contribute to elevated basal superoxide levels, reduced NO release and impaired vascular relaxation in mesenteric resistance arteries of rats fed HS diet.

Author List

Zhu J, Huang T, Lombard JH

MESH terms used to index this publication - Major topics in bold

Acetophenones
Angiotensin II
Animals
Cyclic N-Oxides
Diet, Sodium-Restricted
Fluorescein
Fluorescent Dyes
Male
Mesenteric Arteries
Methacholine Chloride
Microscopy, Fluorescence
NADPH Oxidases
NG-Nitroarginine Methyl Ester
Nitric Oxide
Oxidative Stress
Oxypurinol
Rats
Rats, Sprague-Dawley
Sodium Chloride, Dietary
Spin Labels
Superoxides
Vascular Resistance
Vasoconstriction
Vasodilation
Xanthine
Xanthine Oxidase