Antimicrobial tissue penetration in a rat model of E. coli epididymitis. J Urol 1991 Nov;146(5):1413-7
Date
11/01/1991Pubmed ID
1942312DOI
10.1016/s0022-5347(17)38126-0Scopus ID
2-s2.0-0026334725 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Following induction of unilateral epididymitis by intratesticular injection of E. coli, a single intraperitoneal dose of amdinocillin, ampicillin, doxycycline, tobramycin, or trimethoprim/sulfamethoxazole was administered to five groups of rats. The animal was sacrificed serially and concentrations of antibiotic in serum, infected epididymides, and non-infected epididymides were determined by high performance liquid chromatography. The ratio of infected to non-infected tissue area under the curve values was 1.05 for trimethoprim, 1.58 for sulfamethoxazole, 1.67 for amdinocillin, 2.01 for tobramycin, 2.25 for doxycycline, and 2.58 for ampicillin. Except for trimethoprim, infected tissue concentrations were significantly greater than compared to uninfected epididymal levels (p less than 0.05). Antibiotic concentrations in infected epididymides compared to serum revealed overall penetration of 34% for amdinocillin, 66% for sulfamethoxazole, 70% for ampicillin, 76% for tobramycin, 256% for trimethoprim, and 257% for doxycycline. In a rat model of epididymitis, trimethoprim and doxycycline demonstrated the greatest degree of epididymal penetration compared to serum. All antibiotics except trimethoprim had significantly greater penetration into infected tissue when compared to non-infected epididymal tissue.
Author List
Tartaglione TA, Taylor TO, Opheim KE, See WA, Berger REMESH terms used to index this publication - Major topics in bold
AnimalsAnti-Bacterial Agents
Cell Membrane Permeability
Chromatography, High Pressure Liquid
Disease Models, Animal
Epididymis
Epididymitis
Escherichia coli Infections
Male
Rats
Rats, Inbred Strains
Time Factors
