Congenic mapping and sequence analysis of the Renin locus. Hypertension 2013 Apr;61(4):850-6
Date
03/06/2013Pubmed ID
23460292Pubmed Central ID
PMC3770301DOI
10.1161/HYPERTENSIONAHA.111.01008Scopus ID
2-s2.0-84875210909 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
Renin was the first blood pressure (BP) quantitative trait locus mapped by linkage analysis in the rat. Subsequent BP linkage and congenic studies capturing different portions of the renin region have returned conflicting results, suggesting that multiple interdependent BP loci may be residing in the chromosome 13 BP quantitative trait locus that includes Renin. We used SS-13(BN) congenic strains to map 2 BP loci in the Renin region (chr13: 45.2-49.0 Mb). We identified a 1.1-Mb protective Brown Norway region around Renin (chr13: 46.1-47.2 Mb) that significantly decreased BP by 32 mm Hg. The Renin protective BP locus was offset by an adjacent hypertensive locus (chr13: 47.2-49.0 Mb) that significantly increased BP by 29 mm Hg. Sequence analysis of the protective and hypertensive BP loci revealed 1433 and 2063 variants between Dahl salt-sensitive/Mcwi and Brown Norway rats, respectively. To further reduce the list of candidate variants, we regenotyped an overlapping SS-13(SR) congenic strain (S/renrr) with a previously reported BP phenotype. Sequence comparison among Dahl salt-sensitive, Dahl R, and Brown Norway reduced the number of candidate variants in the 2 BP loci by 42% for further study. Combined with previous studies, these data suggest that at least 4 BP loci reside within the 30-cM chromosome 13 BP quantitative trait locus that includes Renin.
Author List
Flister MJ, Hoffman MJ, Reddy P, Jacob HJ, Moreno CMESH terms used to index this publication - Major topics in bold
AllelesAnimals
Animals, Congenic
Blood Pressure
Chromosome Mapping
Disease Models, Animal
Hypertension
Quantitative Trait Loci
Rats
Rats, Inbred BN
Rats, Inbred Dahl
Renin
Sequence Analysis
