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Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites. Biochemistry 2015 Jul 14;54(27):4163-6

Date

06/27/2015

Pubmed ID

26115234

Pubmed Central ID

PMC4809050

DOI

10.1021/acs.biochem.5b00560

Scopus ID

2-s2.0-84949681845 (requires institutional sign-in at Scopus site)   36 Citations

Abstract

CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.

Author List

Veldkamp CT, Kiermaier E, Gabel-Eissens SJ, Gillitzer ML, Lippner DR, DiSilvio FA, Mueller CJ, Wantuch PL, Chaffee GR, Famiglietti MW, Zgoba DM, Bailey AA, Bah Y, Engebretson SJ, Graupner DR, Lackner ER, LaRosa VD, Medeiros T, Olson ML, Phillips AJ, Pyles H, Richard AM, Schoeller SJ, Touzeau B, Williams LG, Sixt M, Peterson FC

Author

Francis C. Peterson PhD Professor in the Biochemistry department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Binding Sites
Chemokine CCL19
Humans
Membrane Glycoproteins
Models, Molecular
Protein Conformation
Receptors, CCR7