Interleukin-23 Increases Intestinal Epithelial Cell Permeability In Vitro. Eur J Pediatr Surg 2016 Jun;26(3):260-6
Date
05/27/2015Pubmed ID
26007691DOI
10.1055/s-0035-1551563Scopus ID
2-s2.0-84930079309 (requires institutional sign-in at Scopus site) 6 CitationsAbstract
Background Breast milk has a heterogeneous composition that differs between mothers and changes throughout the first weeks after birth. The proinflammatory cytokine IL-23 has a highly variable expression in human breast milk. We hypothesize that IL-23 found in human breast milk is biologically active and promotes epithelial barrier dysfunction. Methods The immature rat small intestinal epithelial cell line, IEC-18, was grown on cell inserts or standard cell culture plates. Confluent cultures were exposed to human breast milk with high or low levels of IL-23 and barrier function was measured using a flux of fluorescein isothiocyanate-dextran (FD-70). In addition, protein and mRNA expression of occludin and ZO-1 were measured and immunofluorescence used to stain occludin and ZO-1. Results Exposure to breast milk with high levels of IL-23 caused an increase flux of FD-70 compared with both controls and breast milk with low levels of IL-23. The protein expression of ZO-1 but not occludin was decreased by exposure to high levels of IL-23. These results correlate with immunofluorescent staining of ZO-1 and occludin which show decreased staining of occludin in both the groups exposed to breast milk with high and low IL-23. Conversely, cells exposed to high IL-23 breast milk had little peripheral staining of ZO-1 compared with controls and low IL-23 breast milk. Conclusion IL-23 in human breast milk is biologically active and negatively affects the barrier function of intestinal epithelial cells through the degradation of tight junction proteins.
Author List
Heinzerling NP, Donohoe D, Fredrich K, Gourlay DM, Liedel JLAuthor
David M. Gourlay MD Chief, Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Line
Cell Membrane Permeability
Dextrans
Epithelial Cells
Female
Fluorescein-5-isothiocyanate
Humans
Interleukin-23
Intestine, Small
Milk, Human
Occludin
RNA, Messenger
Rats
Real-Time Polymerase Chain Reaction
Zonula Occludens-1 Protein
