Faculty Collaboration Database

Homologous up-regulation of the prolactin receptor in rat prostatic explants. Mol Cell Endocrinol 1986 Jun;46(1):53-7

Date

06/01/1986

Pubmed ID

3011565

DOI

10.1016/0303-7207(86)90069-9

Scopus ID

2-s2.0-0022871665 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

Exposure of explants of rat ventral prostates and a rat Leydig cell tumour to ovine prolactin for 20 h caused alterations of the subsequent membrane binding of 125I-human prolactin to an extent and in a direction dependent on the dose of hormone used. Low prolactin concentrations (1-10 micrograms/2 ml) were associated with an increase in binding (up-regulation) which was 75% in the case of the prostatic tissue and 500% in the case of the tumour tissue above control levels. Higher concentrations caused a dose-dependent decrease in binding to below control levels (down-regulation), alterations which could not be explained by receptor occupancy. Time studies with an up-regulatory dose of hormone (3 micrograms/2 ml) indicated that the effects of prolactin on its receptor did not begin to become manifest until after 6-12 h of culture. The results suggest that homologous up-regulation of prolactin binding may be a general feature of prolactin target organs and that explant cultures of prostatic tissue may provide a convenient model for exploring its mechanisms.

Author List

Rui H, Brekke I, Torjesen PA, Purvis K

MESH terms used to index this publication - Major topics in bold

Animals
Culture Techniques
Kinetics
Leydig Cell Tumor
Male
Prolactin
Prostate
Rats
Rats, Inbred Strains
Receptors, Cell Surface
Receptors, Prolactin