B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: a recapitulation of normal B-cell ontogeny. Blood 1990 Oct 15;76(8):1647-56
Date
10/15/1990Pubmed ID
1698484Scopus ID
2-s2.0-0025172230 (requires institutional sign-in at Scopus site) 143 CitationsAbstract
The circulating lymphocytes of 88 consecutive patients following autologous, conventional, or T-cell depleted bone marrow transplantation were serially analyzed for B-cell surface antigen expression and function. In the majority of patients, except for those who developed chronic graft-versus-host disease, the number of circulating CD20+ B cell normalized by the fourth posttransplant month. The earliest detectable B cells normally expressed HLA-DR, CD19, surface immunoglobulin (slg), CD21, Leu-8, and lacked expression of CD10 (CALLA). In addition, the circulating B cells expressed CD1c, CD38, CD5, and CD23 for the first year following transplant, antigens that are normally expressed on a small percentage of circulating B cells in normal adults, but highly expressed on cord blood B cells. Similar to cord blood B cells, patient B cells isolated during the first year following transplant, proliferated normally to Staphylococcus aureus Cowan strain I (SAC), and produced IgM, but minimal or no IgG when stimulated with pokeweed mitogen and SAC, unlike normal adult B cells that produce both. The similar phenotype and function of posttransplant and cord blood B cells, and their similar rate of decline in patients and normal children adds further evidence to support the hypothesis that B-cell differentiation posttransplant is recapitulating normal B-cell ontogeny.
Author List
Small TN, Keever CA, Weiner-Fedus S, Heller G, O'Reilly RJ, Flomenberg NMESH terms used to index this publication - Major topics in bold
AdultAging
Antigens, CD
Antigens, CD20
Antigens, Differentiation, B-Lymphocyte
B-Lymphocytes
Bone Marrow Transplantation
Cell Differentiation
Child
Child, Preschool
Graft vs Host Disease
HLA-DR Antigens
Humans
Immunoglobulin G
Immunoglobulin M
Immunophenotyping
Infant, Newborn
Leukocyte Count
Lymphocyte Activation
Staphylococcus aureus
T-Lymphocytes
