Co-activation of atrial natriuretic factor promoter by Tip60 and serum response factor. J Biol Chem 2006 Jun 02;281(22):15082-9
Date
04/07/2006Pubmed ID
16597624DOI
10.1074/jbc.M513593200Scopus ID
2-s2.0-33744956273 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
Tat-interactive protein 60 (Tip60) is a member of the MYST family of histone acetyltransferases (HATs). In addition to its HAT domain, Tip contains a heterochromatin-associated protein 1-like chromodomain and a zinc finger-like domain. Several alternative splice variants of Tip60 have been characterized, including full-length Tip60alpha, Tip60beta (which lacks exon V encoded by the Tip60 gene), and Tip55 (which encodes a novel 103-amino-acid C terminus). We report here that isoproteins recognized by a pan-Tip60 antibody are strongly and transiently expressed between embryonic days 8 and 11 in the embryonic mouse myocardium. A functional role for Tip60 isoproteins in cardiac myocyte differentiation is suggested by immunoprecipitation experiments showing that Tip60alpha, Tip60beta, and Tip55 can bind serum response factor (SRF) and by transient transfection assessments showing that Tip60 and SRF cooperatively activate the atrial natriuretic factor promoter. Although this combinatorial activity is inhibited by histone deacetylase 7, it was unexpectedly enhanced by point mutation of the HAT domain. Ablation of the chromodomain from Tip60beta caused derepression. These findings suggest that Tip60 modulates expression of SRF-dependent cardiac genes.
Author List
Kim MS, Merlo X, Wilson C, Lough JMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Atrial Natriuretic Factor
Base Sequence
Cell Differentiation
DNA
Fetal Heart
Gene Expression Regulation
HeLa Cells
Histone Acetyltransferases
Humans
Mice
Molecular Sequence Data
Myocytes, Cardiac
Point Mutation
Promoter Regions, Genetic
Recombinant Proteins
Serum Response Factor
Transfection
