Response of radiation-induced fibrosarcoma-1 in mice to cyclophosphamide monitored by in vivo 31P nuclear magnetic resonance spectroscopy. Cancer Res 1988 Sep 01;48(17):4736-42
Date
09/01/1988Pubmed ID
3409214Scopus ID
2-s2.0-0023812995 (requires institutional sign-in at Scopus site) 53 CitationsAbstract
In vivo 31P nuclear magnetic resonance spectroscopy has been used to examine the RIF-1 fibrosarcoma in mice during untreated growth and following chemotherapy with cyclophosphamide. Levels of inorganic phosphate increase relative to phosphocreatine or nucleoside triphosphates during early untreated growth. After the tumor reaches a volume of approximately 1 g, no further decrease in energy level is observed. Following treatment with cyclophosphamide, tumor phosphorus metabolite ratios and pH are significantly altered, compared to untreated age-matched controls. During the growth delay period following chemotherapy there is a significant reduction in the ratio of inorganic phosphate to other phosphate metabolites, compared to age-matched controls. In addition, a more alkaline pH is observed in the tumors of treated animals. When the growth delay period ends, nuclear magnetic resonance spectra return to pretreatment patterns. The magnitude of the differences in 31P nuclear magnetic resonance spectral parameters between treated animals and untreated controls is dose dependent. However, doses of cyclophosphamide above 200 mg/kg do not result in earlier spectroscopic alterations, nor in larger effects by Day 3 after treatment, even though clonogenic cell killing and growth delay are greater at these higher doses.
Author List
Li SJ, Wehrle JP, Rajan SS, Steen RG, Glickson JD, Hilton JAuthor
Shi-Jiang Li PhD, MS Emeritus Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCyclophosphamide
Dose-Response Relationship, Drug
Fibrosarcoma
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Mice
Mice, Inbred C3H
Neoplasms, Radiation-Induced
Phosphates
Phosphocreatine
