Faculty Collaboration Database

Recovery of BK virus large T-antigen-specific cellular immune response correlates with resolution of bk virus nephritis. Transplantation 2008 Jan 27;85(2):185-92

Date

01/24/2008

Pubmed ID

18212622

DOI

10.1097/TP.0b013e31815fef56

Scopus ID

2-s2.0-38549110496 (requires institutional sign-in at Scopus site)   58 Citations

Abstract

BACKGROUND: BK virus (BKV) infection of kidney transplant patients is an increasing problem and is thought to be secondary to potent immunosuppressive therapy. BKV infection progresses to BKV nephritis (BKVN) in approximately 8% of transplants and in half of these cases the graft is lost.

METHODS: We used an interferon-gamma enzyme-linked immunosorbent spot (ELISPOT) assay to measure the cellular immune response to peptides encoding BKV large T antigen. Eight kidney transplant patients with BKVN were tested at the time of diagnosis of BKVN and then after resolution of active BKV infection.

RESULTS: When total spot counts from all peptide pools were combined, the mean ELISPOT signal per 10,000 cells at the time of BKVN diagnosis was 23.1 (range 3.4-59.7), with a median of 21.8. This increased to 70.2 (range 5.4-189.4) with a median of 37.0 (P=0.1216) after resolution of active BKV infection. To further increase specificity of response, we counted the number of peptide pools with ELISPOT activity of greater than 10 spots per well after subtraction of background. The mean number of pools fitting this criteria at the time of BKVN diagnosis was 2.1 (range 0-8) with a median of 1.5; this increased to 8 (range 1-18) and a median of 6.5 after recovery (P=0.0338).

CONCLUSION: This demonstrates that recovery of cellular immune response to large T antigen corresponds with resolution of active BKV infection. This may prove useful in monitoring patients' cellular immunity and recovery from active BKV infection when treated with reduction in immunosuppressive therapy.

Author List

Prosser SE, Orentas RJ, Jurgens L, Cohen EP, Hariharan S

MESH terms used to index this publication - Major topics in bold

Adult
Antigens, Polyomavirus Transforming
BK Virus
Drug Therapy, Combination
Female
Humans
Immunity, Cellular
Immunosuppressive Agents
Kidney Transplantation
Male
Middle Aged
Nephritis
Polyomavirus Infections
Postoperative Complications
Treatment Outcome
Tumor Virus Infections