Faculty Collaboration Database

Small vessel membrane potential, sympathetic input, and electrogenic pump rate in SHR. Am J Physiol 1986 Apr;250(4 Pt 1):C547-56

Date

04/01/1986

Pubmed ID

3008565

DOI

10.1152/ajpcell.1986.250.4.C547

Scopus ID

2-s2.0-0022515762 (requires institutional sign-in at Scopus site)   53 Citations

Abstract

Comparative measurements of transmembrane potential (Em) were made in situ in vascular smooth muscle cells (VSM) of mesenteric small principal arteries and veins with innervation and circulation intact. Vessels were in an externalized, topically suffused jejunal loop in 4- to 5-wk-old (initial hypertension) and 12- to 15-wk-old (established hypertension) anesthetized, spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) normotensive control rats. Comparable in vitro measurements of Em were also made in VSM of isolated intact small mesenteric vessel segments (from the 12- to 15-wk-old animals) maintained at their in situ lengths and suffused with physiological salt solution (PSS). During suffusion in situ with control PSS, VSM of both small veins and arteries in older (but not younger)SHR were less polarized than in WKY. Local chemical sympathetic denervation in situ (with 6-hydroxydopamine) hyperpolarized VSM of both vessel types in older (but not younger) SHR to the same Em levels measured in situ in respective WKY vessels. After local denervation, VSM of small arteries (but not veins) of both SHR and WKY remained less polarized in situ than in vitro, suggesting the presence of one or more circulating factors with a specific depolarizing action on the arterial side in both animal types. In vitro, VSM of both small arteries and veins from WKY but not SHR were depolarized immediately by 10(-3) M ouabain. In contrast, reduction of the PSS suffusate temperature to 16 degrees C caused a significantly greater depolarization in VSM of SHR vessels.(ABSTRACT TRUNCATED AT 250 WORDS)

Author List

Stekiel WJ, Contney SJ, Lombard JH

MESH terms used to index this publication - Major topics in bold

Aging
Animals
Blood Pressure
Denervation
Heart Rate
Hydroxydopamines
Male
Membrane Potentials
Microcirculation
Muscle, Smooth, Vascular
Oxidopamine
Potassium
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Sodium-Potassium-Exchanging ATPase
Splanchnic Circulation
Sympathetic Nervous System
Temperature
Time Factors