Mapping the Cell-Surface N-Glycoproteome of Human Hepatocytes Reveals Markers for Selecting a Homogeneous Population of iPSC-Derived Hepatocytes. Stem Cell Reports 2016 Sep 13;7(3):543-556
Date
08/30/2016Pubmed ID
27569060Pubmed Central ID
PMC5032032DOI
10.1016/j.stemcr.2016.07.016Scopus ID
2-s2.0-84990058399 (requires institutional sign-in at Scopus site) 39 CitationsAbstract
When comparing hepatic phenotypes between iPSC-derived hepatocyte-like cells from different liver disease patients, cell heterogeneity can confound interpretation. We proposed that homogeneous cell populations could be generated by fluorescence-activated cell sorting (FACS). Using cell-surface capture proteomics, we identified a total of 300 glycoproteins on hepatocytes. Analyses of the expression profiles during the differentiation of iPSCs revealed that SLC10A1, CLRN3, and AADAC were highly enriched during the final stages of hepatocyte differentiation. FACS purification of hepatocyte-like cells expressing SLC10A1, CLRN3, or AADAC demonstrated enrichment of cells with hepatocyte characteristics. Moreover, transcriptome analyses revealed that cells expressing the liver gene regulatory network were enriched while cells expressing a pluripotent stem cell network were depleted. In conclusion, we report an extensive catalog of cell-surface N-linked glycoproteins expressed in primary hepatocytes and identify cell-surface proteins that facilitate the purification of homogeneous populations of iPSC-derived hepatocyte-like cells.
Author List
Mallanna SK, Cayo MA, Twaroski K, Gundry RL, Duncan SAMESH terms used to index this publication - Major topics in bold
BiomarkersCell Differentiation
Cluster Analysis
Hepatocytes
Humans
Induced Pluripotent Stem Cells
Membrane Glycoproteins
Organ Specificity
Phenotype
Proteome
Proteomics
