Immunologic response to Faenia rectivirgula (Micropolyspora faeni) in a dairy farm family. J Allergy Clin Immunol 1988 Aug;82(2):190-5
Date
08/01/1988Pubmed ID
3042838DOI
10.1016/0091-6749(88)90998-0Scopus ID
2-s2.0-0023737087 (requires institutional sign-in at Scopus site) 12 CitationsAbstract
In the present study, cellular and humoral responses to Faenia rectivirgula antigens were evaluated in seven subjects, members of a family who lived and worked on a dairy farm. Four subjects had clinical features of hypersensitivity pneumonitis after exposure to moldy hay. The other three subjects had no clinical disease in spite of similar exposure. Although serum precipitins were found in most subjects, a biotin-avidin-linked immunosorbent assay revealed high levels of F. rectivirgula-specific antibodies only in the symptomatic subjects. In addition, numerous precipitin arcs were present in the sera of the symptomatic but not the asymptomatic subjects by antigen-antibody crossed immunoelectrophoresis. No clear distinction between symptomatic and asymptomatic subjects could be made on the basis of lymphocyte phenotype studies, and antigen-induced lymphocyte transformation was not detected in any subjects. The results indicate that F. rectivirgula-specific antibody levels as detected by biotin-avidin-linked immunosorbent assay and by the presence of precipitin arcs in crossed immunoelectrophoresis may differentiate symptomatic and asymptomatic farmers.
Author List
Brummund W, Kurup VP, Resnick A, Milson TJ Jr, Fink JNMESH terms used to index this publication - Major topics in bold
Actinomycetales InfectionsAntibody Formation
Antigens, Differentiation, B-Lymphocyte
Antigens, Differentiation, T-Lymphocyte
Dairying
Farmer's Lung
Humans
Immunoelectrophoresis, Two-Dimensional
Immunosorbent Techniques
Micromonosporaceae
Phenotype
Precipitin Tests
