Isolation and characterization of a Neurospora glucose-repressible gene. Curr Genet 1988 Dec;14(6):545-51
Date
12/01/1988Pubmed ID
2977301DOI
10.1007/BF00434079Scopus ID
2-s2.0-0024271454 (requires institutional sign-in at Scopus site) 89 CitationsAbstract
Using differential hybridization, the cDNA copy of a Neurospora gene coding for an abundant glucose-repressible mRNA (grg-1) has been isolated. The cDNA was used to clone the genomic copy, and both were sequenced. The cDNA is nearly full length and contains putative translational start and termination codons. Conceptual translation indicates that grg-1 mRNA could direct the synthesis of a 7,000 molecular weight polypeptide. The genomic clone, contained in an 1,888 bp PvuII fragment, encompasses the entire cDNA as well as 838 bp of 5' and 369 bp of 3' flanking sequence. Comparison of the cDNA and genomic clones revealed the presence of two short introns in potential protein-coding sequences. grg-1 message levels were found to increase within minutes following the onset of glucose deprivation and rise 50 fold during the first 90 min of derepression.
Author List
McNally MT, Free SJMESH terms used to index this publication - Major topics in bold
Base SequenceBlotting, Northern
Blotting, Southern
Cloning, Molecular
DNA Probes
DNA, Fungal
Gene Expression Regulation
Genes, Fungal
Glucose
Molecular Sequence Data
Neurospora
Neurospora crassa
Nucleic Acid Hybridization
RNA, Fungal
RNA, Messenger
Repressor Proteins
Restriction Mapping
Transcription Factors
Transcription, Genetic
