Faculty Collaboration Database

Localization of the Kv1.5 K+ channel protein in explanted cardiac tissue. J Clin Invest 1995 Jul;96(1):282-92

Date

07/01/1995

Pubmed ID

7615797

Pubmed Central ID

PMC185199

DOI

10.1172/JCI118032

Scopus ID

2-s2.0-0029082517 (requires institutional sign-in at Scopus site)   194 Citations

Abstract

The cloned Kv1.5 K+ channel displays similar kinetics and pharmacology to a delayed rectifier channel found in atrial myocytes. To determine whether the Kv1.5 isoform plays a role in the cardiac action potential, it is necessary to confirm the expression of this channel in cardiac myocytes. Using antibodies directed against two distinct channel epitopes, the Kv1.5 isoform was localized in human atrium and ventricle. Kv1.5 was highly localized at intercalated disk regions as determined by colocalization with connexin and N-cadherin specific antibodies. While both antichannel antibodies localized the Kv1.5 protein in cardiac myocytes, only the NH2-terminal antibodies stained vascular smooth muscle. The selective staining of vasculature by this antiserum suggests that epitope accessibility, and perhaps channel structure, varies between cardiac and vascular myocytes. Kv1.5 expression was localized less in newborn tissue, with punctate antibody staining dispersed on the myocyte surface. This increasing organization with age was similar to that observed for connexin. Future work will address whether altered K+ channel localization is associated with cardiac disease in addition to changing with development.

Author List

Mays DJ, Foose JM, Philipson LH, Tamkun MM

MESH terms used to index this publication - Major topics in bold

Amino Acid Sequence
Animals
Base Sequence
Cell Communication
Humans
Infant, Newborn
Kv1.5 Potassium Channel
Male
Molecular Sequence Data
Muscle, Smooth, Vascular
Myocardium
Potassium Channels
Potassium Channels, Voltage-Gated
Rats
Rats, Sprague-Dawley