Renal AT1 receptor protein expression during the early stage of diabetes mellitus. Int J Exp Diabetes Res 2002;3(2):97-108
Date
05/07/2002Pubmed ID
11991202Pubmed Central ID
PMC2478573DOI
10.1080/15604280214483Scopus ID
2-s2.0-0036012687 (requires institutional sign-in at Scopus site) 35 CitationsAbstract
Experiments were performed to evaluate the hypothesis that the early stage of Type 1 diabetes mellitus (DM) increases renal angiotensin II (AngII) concentration and angiotensin type 1 (AT) receptor protein levels. Nineteen or twenty days after vehicle (Sham rats) or streptozotocin (STZ rats) treatment, plasma [AngII] was higher in STZ rats (152 +/- 23 fmol/ml) than in Sham rats (101 +/- 7 fmol/ml); however, kidney [AngII] did not differ between groups. AT1 receptor protein expression was greater in STZ kidneys than in Sham kidneys. This increase was restricted to the cortex, where AT1 protein levels were elevated by 77 +/- 26% (42 kDa) and 101 +/- 16% (58 kDa) in STZ kidneys. Immunohistochemistry revealed this effect to be most evident in distal nephron segments including the connecting tubule/cortical collecting duct. Increased renal cortical AT1 receptor protein and circulating AngII levels are consistent with an exaggerated AngII-dependent influence on renal function during the early stage of DM in the rat.
Author List
Harrison-Bernard LM, Imig JD, Carmines PKMESH terms used to index this publication - Major topics in bold
Angiotensin IIAnimals
Blood Glucose
Blotting, Western
Delayed-Action Preparations
Diabetes Mellitus, Experimental
Drug Implants
Hypoglycemic Agents
Insulin
Kidney
Male
Protein Isoforms
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1
Receptors, Angiotensin
Reference Values
