Faculty Collaboration Database

Quantitative imaging of lymph function. Am J Physiol Heart Circ Physiol 2007 Jun;292(6):H3109-18

Date

02/20/2007

Pubmed ID

17307997

DOI

10.1152/ajpheart.01223.2006

Scopus ID

2-s2.0-34447528871 (requires institutional sign-in at Scopus site)   107 Citations

Abstract

Functional lymphatic imaging was demonstrated in the abdomen and anterior hindlimb of anesthetized, intact Yorkshire swine by using near-infrared (NIR) fluorescence imaging following intradermal administration of 100-200 microl of 32 microM indocyanine green (ICG) and 64 microM hyaluronan NIR imaging conjugate to target the lymph vascular endothelial receptor (LYVE)-1 on the lymph endothelium. NIR fluorescence imaging employed illumination of 780 nm excitation light ( approximately 2 mW/cm(2)) and collection of 830 nm fluorescence generated from the imaging agents. Our results show the ability to image the immediate trafficking of ICG from the plexus, through the vessels and lymphangions, and to the superficial mammary, subiliac, and middle iliac lymph nodes, which were located as deep as 3 cm beneath the tissue surface. "Packets" of ICG-transited lymph vessels of 2-16 cm length propelled at frequencies of 0.5-3.3 pulses/min and velocities of 0.23-0.75 cm/s. Lymph propulsion was independent of respiration rate. In the case of the hyaluronan imaging agent, lymph propulsion was absent as the dye progressed immediately through the plexus and stained the lymph vessels and nodes. Lymph imaging required 5.0 and 11.9 microg of ICG and hyaluronan conjugate, respectively. Our results suggest that microgram quantities of NIR optical imaging agents and their conjugates have a potential to image lymph function in patients suffering from lymph-related disorders.

Author List

Sharma R, Wang W, Rasmussen JC, Joshi A, Houston JP, Adams KE, Cameron A, Ke S, Kwon S, Mawad ME, Sevick-Muraca EM

Author

Amit Joshi PhD Professor in the Biomedical Engineering department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Abdomen
Animals
Endothelium, Lymphatic
Feasibility Studies
Fluorescent Dyes
Hindlimb
Hyaluronan Receptors
Hyaluronic Acid
Image Interpretation, Computer-Assisted
Indocyanine Green
Injections, Intradermal
Lymph
Lymphatic Diseases
Lymphatic System
Pulsatile Flow
Spectrometry, Fluorescence
Spectroscopy, Near-Infrared
Swine
Time Factors