A human anti-Pseudomonas aeruginosa serotype O6ad immunoglobulin G1 expressed in transgenic tobacco is capable of recruiting immune system effector function in vitro. Antimicrob Agents Chemother 2007 Sep;51(9):3322-8
Date
07/04/2007Pubmed ID
17606688Pubmed Central ID
PMC2043195DOI
10.1128/AAC.00366-07Scopus ID
2-s2.0-35948970963 (requires institutional sign-in at Scopus site) 23 CitationsAbstract
The production of a recombinant human IgG1 in transgenic tobacco was examined to determine whether a plant-derived antibody could recruit immune system effector function against a bacterial pathogen. A plant transformation vector was engineered to contain genes for a human kappa light chain and a human gamma-1 heavy chain with V(H) and V(L) sequences from a previously identified human IgG2 monoclonal antibody (MAb) that specifically binds to and opsonizes Pseudomonas aeruginosa serotype O6ad. Unique NcoI and NotI restriction sites were incorporated to flank these variable sequences, resulting in a plant transformation vector that could be engineered for expression of any other human IgG1 antibody, requiring only the substitution of other V(H) and V(L) antigen-binding coding sequences. The plant-produced IgG1 was determined to have high-mannose glycan content and to be capable of mediating opsonophagocytosis of P. aeruginosa serotype O6ad in vitro using human complement and human polymorphonuclear leukocytes. Thus, MAbs produced in plants from this vector could provide human IgG1 MAbs for targeting other pathogens that require the recruitment of immune system effector functions.
Author List
McLean MD, Almquist KC, Niu Y, Kimmel R, Lai Z, Schreiber JR, Hall JCMESH terms used to index this publication - Major topics in bold
Antibodies, MonoclonalEndoplasmic Reticulum
Genetic Vectors
Humans
Immune System
Immunoglobulin G
Immunoglobulin Heavy Chains
In Vitro Techniques
Mannose
Molecular Sequence Data
Neutrophils
Opsonin Proteins
Phagocytosis
Plants, Genetically Modified
Polysaccharides
Pseudomonas aeruginosa
Recombinant Proteins
