T-cell hybridomas from HLA-transgenic mice as tools for analysis of human antigen processing. J Immunol Methods 2003 Oct 01;281(1-2):129-42
Date
10/29/2003Pubmed ID
14580887DOI
10.1016/j.jim.2003.07.004Scopus ID
2-s2.0-0142216515 (requires institutional sign-in at Scopus site) 40 CitationsAbstract
The study of antigen processing and presentation by human antigen presenting cells (APC) has been limited by difficulties of producing and maintaining human T-cell clones. Murine T-cell hybridomas have advantages for detecting specific peptide-MHC complexes on APC. Human antigen-specific immortalized T-cell lines have not been successfully produced. We report and validate the use of transgenic mice with human MHC genes for HLA-A2, DR1 and DR4 to produce murine T-cell hybridomas that are restricted to human HLA alleles and respond to human macrophages, dendritic cells (DC), and B-cell lines. Hybridomas restricted by human MHC-I and -II specific for influenza matrix protein, tetanus toxoid, diphtheria antigen CRM(197), and various M. tuberculosis antigens were produced. Epitope specificity was determined for several hybridomas. T hybridomas recognized peptide-MHC complexes on fixed APC for analysis of kinetics or susceptibility to inhibitors of antigen processing. T hybridomas restricted by human MHC represent convenient and powerful tools for the study of antigen processing by human APC.
Author List
Canaday DH, Gehring A, Leonard EG, Eilertson B, Schreiber JR, Harding CV, Boom WHMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Antigen Presentation
Antigen-Presenting Cells
Cell Line
Epitope Mapping
HLA-A2 Antigen
Humans
Hybridomas
Interferon-gamma
Mice
Mice, Transgenic
Molecular Sequence Data
T-Lymphocytes
