Faculty Collaboration Database

Significant variation in serotype-specific immunogenicity of the seven-valent Streptococcus pneumoniae capsular polysaccharide-CRM197 conjugate vaccine occurs despite vigorous T cell help induced by the carrier protein. J Infect Dis 2003 May 15;187(10):1629-38

Date

05/02/2003

Pubmed ID

12721943

DOI

10.1086/374785

Scopus ID

2-s2.0-0037737872 (requires institutional sign-in at Scopus site)   65 Citations

Abstract

Streptococcus pneumoniae capsular polysaccharides (PnPSs) induce protective antibodies but are T cell-independent type 2 antigens and are poorly immunogenic in infants. Conjugate vaccines of PnPSs linked to proteins like cross-reactive material (CRM(197)) increase PS antibody titer and elicit immunologic memory in infants. Despite being linked to an identical carrier protein, each PS component of the 7-valent PnPS-CRM(197) vaccine has different immunogenicity. To determine whether variations in conjugate-induced memory T cell responses or PnPS-specific antibody-secreting cells (ASCs) were responsible for serotype-specific differences in immunogenicity, adults were immunized with 7-valent PnPS-CRM(197), and antibody titer, vaccine component-specific CD4(+) T cell recall response, numbers of PnPS-specific ASCs, and cytokine production were measured. PnPS-CRM(197) induced significantly different serotype-specific antibody titers, despite vigorous T cell recall responses to all 7 vaccine components, and production of interleukin (IL)-2, IL-5, IL-6, IL-10, and interferon-gamma. We conclude that PnPS-CRM(197) induces variable serotype-specific antibody titers, despite induction of comparable CRM(197)-specific memory T cell responses.

Author List

Kamboj KK, Kirchner HL, Kimmel R, Greenspan NS, Schreiber JR

MESH terms used to index this publication - Major topics in bold

Adult
Antibodies, Bacterial
Antigens, Bacterial
Bacterial Capsules
Bacterial Proteins
CD4-Positive T-Lymphocytes
Cytokines
Female
Humans
Immunologic Memory
Male
Pneumococcal Vaccines
Serotyping
Streptococcus pneumoniae
Th1 Cells
Th2 Cells
Time Factors
Vaccines, Conjugate