Faculty Collaboration Database

Immunization with Haemophilus influenzae type b-CRM(197) conjugate vaccine elicits a mixed Th1 and Th2 CD(4+) T cell cytokine response that correlates with the isotype of antipolysaccharide antibody. J Infect Dis 2001 Oct 01;184(7):931-5

Date

08/31/2001

Pubmed ID

11528593

DOI

10.1086/323342

Scopus ID

2-s2.0-0035479187 (requires institutional sign-in at Scopus site)   45 Citations

Abstract

Haemophilus influenzae type b (Hib) capsular polysaccharide (PS) induces protective antibodies but is T independent and poorly immunogenic in infants. Conjugate vaccines of Hib PS linked to proteins, such as CRM(197), increase the PS antibody titer and elicit immunologic memory. To define the conjugate-induced memory T cell response, 19 adults were immunized with Hib-CRM(197), and antibody titers, carrier protein-specific CD4(+) T cell proliferation, and cytokine production were measured. Hib-CRM(197) induced PS and CRM(197) antibodies, vigorous T cell recall responses, and production of cytokines, including interleukin (IL)-2, IL-5, IL-10, and interferon-gamma. There was marked variability in PS antibody titer, despite consistent CRM(197)-specific recall responsiveness, which correlated with peak IgM and IgA PS antibody titers. Correlations were also found between IL-2 and IL-5 and IgA PS antibody levels. Hib-CRM(197) induced a rapid increase in CRM(197)-specific memory T cells and mixed Th1/Th2 cytokines, which may regulate the isotype and quantity of PS antibody.

Author List

Kamboj KK, King CL, Greenspan NS, Kirchner HL, Schreiber JR

MESH terms used to index this publication - Major topics in bold

Adult
Antibodies, Bacterial
Bacterial Capsules
Bacterial Proteins
Cytokines
Haemophilus Infections
Haemophilus Vaccines
Haemophilus influenzae type b
Humans
Immunoglobulin A
Immunoglobulin M
Immunologic Memory
Injections, Subcutaneous
Interferon-gamma
Interleukins
Polysaccharides, Bacterial
Th1 Cells
Th2 Cells
Vaccination
Vaccines, Conjugate